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KMID : 1161520210250030152
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Animal Cells and Systems
2021 Volume.25 No. 3 p.152 ~ p.160
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MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2
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Shayimu Paerhati
Yusufu Aikeremu
Rehemutula Aizimaiti
Redati Darebai
Jiapaer Rexida
Tuerdi Rousidan
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Abstract
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Colon cancer is a malignant tumor that seriously affects human health. Recently, studies revealed that the expression of MTBP enhanced the proliferation and metastasis of many types of cancer cells. And the data also showed that MTBP has the potential to regulate the expression of ZEB2. However, it is unclear whether MTBP can affect the proliferation, migration and invasion of colon cancer cells by modulating the expression of ZEB2. In this study, we established the MTBP overexpression and knockdown colon cancer cells with the transfection. Next, CCK-8 and transwell assays were carried out to determine the changes of the proliferation and invasion of colon cancer cells, respectively. After that, we overexpressed the ZEB2 in these MTBP knockdown colon cancer cells. Finally, the invasion and migration of these cells were detected with the same methods. We revealed that overexpression of MTBP enhanced the proliferation and invasion of colon cancer cells. Moreover, suppression of MTBP repressed the proliferation, migration and invasion of colon cancer cells. Furthermore, MTBP promoted the expression of ZEB2. The overexpression of ZEB2 abolished the MTBP knockdown induced inhibition of the migration and invasion of colon cancer cells. These results implied that MTBP enhanced the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2.
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KEYWORD
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Colon cancer cells, MTBP, ZEB2, proliferation, invasion
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